The Drosophila retinoblastoma binding protein 6 family member has two isoforms and is potentially involved in embryonic patterning.

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dc.contributor.author Hull, R.
dc.contributor.author Oosthuysen, B.
dc.contributor.author Cajee, U.-F.
dc.contributor.author Mokgohloa, L.
dc.contributor.author Nweke, E.
dc.contributor.author Antunes, R.J.
dc.contributor.author Coetzer, T.H.T.
dc.contributor.author Ntwasa, M.
dc.date.accessioned 2016-09-28T15:05:35Z
dc.date.available 2016-09-28T15:05:35Z
dc.date.issued 2015-05
dc.identifier.citation Hull, R. et al. 2015. The Drosophila retinoblastoma binding protein 6 family member has two isoforms and is potentially involved in embryonic patterning. International Journal of Molecular Sciences 16(5), pp.10242-10266. en_ZA
dc.identifier.issn 1661-6596 (Print)
dc.identifier.issn 1422-0067
dc.identifier.uri http://hdl.handle.net/10539/21092
dc.description.abstract The human retinoblastoma binding protein 6 (RBBP6) is implicated in esophageal, lung, hepatocellular and colon cancers. Furthermore, RBBP6 was identified as a strong marker for colon cancer prognosis and as a predisposing factor in familial myeloproliferative neoplasms. Functionally, the mammalian protein interacts with p53 and enhances the activity of Mdm2, the prototypical negative regulator of p53. However, since RBBP6 (known as PACT in mice) exists in multiple isoforms and pact−/− mice exhibit a more severe phenotype than mdm2−/− mutants, it must possess some Mdm2-independent functions. The function of the invertebrate homologue is poorly understood. This is complicated by the absence of the Mdm2 gene in both Drosophila and Caenorhabditis elegans. We have experimentally identified the promoter region of Snama, the Drosophila homologue, analyzed potential transcription factor binding sites and confirmed the existence of an additional isoform. Using band shift and co-immunoprecipitation assays combined with mass spectrometry, we found evidence that this gene may be regulated by, amongst others, DREF, which regulates hundreds of genes related to cell proliferation. The potential transcription factors for Snama fall into distinct functional groups, including anteroposterior embryonic patterning and nucleic acid metabolism. Significantly, previous work in mice shows that pact−/− induces an anteroposterior phenotype in embryos when rescued by simultaneous deletion of p53. Taken together, these observations indicate the significance of RBBP6 proteins in carcinogenesis and in developmental defects. en_ZA
dc.language.iso en en_ZA
dc.publisher MDPI AG en_ZA
dc.subject retinoblastoma binding protein 6 en_ZA
dc.subject RBBP6 en_ZA
dc.subject SNAMA en_ZA
dc.subject p53 en_ZA
dc.subject Rb en_ZA
dc.subject Mdm2 en_ZA
dc.title The Drosophila retinoblastoma binding protein 6 family member has two isoforms and is potentially involved in embryonic patterning. en_ZA
dc.type Article en_ZA
dc.journal.volume 16 en_ZA
dc.journal.title International Journal of Molecular Sciences en_ZA
dc.description.librarian SP2016 en_ZA
dc.citation.doi 10.3390/ijms160510242 en_ZA
dc.citation.epage 10266 en_ZA
dc.citation.issue 5 en_ZA
dc.citation.spage 10242 en_ZA


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