The influence of fatty acids in vitro on mammalian cells from species differing in their fatty acyl desaturase capabilities.
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Date
1991
Authors
Giangregorio, Alfredo
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Journal ISSN
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Abstract
Numerous studies have assessed the effects of single
fatty acids on various aspects of lipid metabolism,
particularly cancer. Established cell lines have largely
been used for this purpose. The choice of control cells.
however, has often been inappropriate. There is also a
surprising lack of knowledge of the effects of fatty
acids in the "real world", in which normal cells in vivo
are presented with mixtures of dietary fatty acids.
Before transformed cells can be used as models of
disease states, it is essential to fully understand
fatty acid metabolism in normal (control) cells. Only
then can experimental findings be extrapolated to the
clinical situation with some certainty. This thesis has
therefore, assessed the effects of exogenous fatty acid
mixtures on the growth/viability of normal mammalian
tissues all cultured under standard conditions, and
attempted to elucidate the mechanisms underlying such
effects.
As different mammalian species exhibit different fatty
acyl desaturase capabilities, cells from three species
were chosen, viz. rat, Man and cat, with desaturase
capability decreasing with species, respectively. A wide
range of different cell types from each species were
studied due to the known differences in their lipid ( Abbreviation abstract )
Description
A Thesis submitted to the Faculty of Medicine,
in Fulfilment of the Requirements for the Degree of Doctor of Philosophy
Keywords
Fatty Acids.