Molecular characterisation of Hepatitis B virus vaccine escape mutants in South Africa
Date
2006-11-17T12:51:16Z
Authors
Crowther, Penny
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Abstract
Since the introduction of vaccination against hepatitis B virus (HBV) infection in South
Africa, at least one case of infection despite vaccination has occurred. The purpose of this
study was to determine whether this infection was the result of mutations within the region
of the surface (S) gene encoding the a determinant epitopes of the hepatitis B surface
antigen, which permitted viral vaccine-escape. HBV DNA was extracted from the serum
and liver tissue of the patient and amplified within the complete 3 215 bp genome and S
gene, respectively. Following cloning, sequencing revealed a minor population displaying
unique or uncommon S gene mutations that resulted in C138R, C139R, K141R, P142L,
T143A, N146D, and T148A amino acid substitutions in the clones from the serum, and
C139Y and D144N in the clones from the liver. Such isolates may represent South African
HBV vaccine-escape mutants that caused chronic infection in the host prior to their
reversion to wild-type.
Description
Student Number : 9903144J -
MSc (Med) dissertation -
Faculty of Health Sciences
Keywords
hepatitis B virus, vaccination, HBV, South Africa, mutations, surface (S) gene encoding, a determinant epitopes, sequencing